RRC-MMPF: New Instruments

From RRC
Jump to: navigation, search


April 2012

LTQ Orbitrap Velos upgraded

Our LTQ Orbitrap Velos has been upgraded to an LTQ Orbitrap Velos Pro. Thermo reports this will give us twice the scan speed and twice the sensitivity.

Please contact Rod Davis (rgdavis_at_uic.edu) with questions about the new capabilities!

July 2011

LTQ Orbitrap Velos has been installed!!!

The LTQ OrbiTrap Velos has been installed and is being testing by the MMPF staff.

For information on gaining access to any of our instruments, please email Rod Davis (rgdavis_at_uic.edu).

April 2011

LTQ Orbitrap Velos is coming soon!!!

UIC has been awarded an NCRR Shared Instrument grant (S10) for the purchase of a Thermo Orbitrap Velos. The grant was awarded to Dr. Richard van Breeemen, Professor of Medicinal Chemistry and Pharmacognosy and Assistant to the RRC Director. Please watch this website for additional details about the timing of installation. Here is a description from Thermo's website

"Thermo Scientific* LTQ Orbitrap Velos provides ultra-high resolution and accurate mass for confident structural elucidation powered by Orbitrap technology. The LTQ Orbitrap Velos combines the industry-leading Orbitrap mass analyzer, a new Higher-Energy Collisional Dissociation (HCD) cell and the new LTQ Velos, the world's fastest and most sensitive ion trap mass spectrometer, to routinely deliver ultra-high resolution and accurate mass data." (ThermoScientific.com)

Check back for updates!!

March 2011

Shimazdu LC20ADXR UFLC pumps

The LC pumps attached to the Shimazdu IT-TOF were recently updated to the UFLC-XR model, which are capable of higher pressure (9,600 psi) liquid chromatography than before. With this increase in capabilities comes an increase in vigilance required. The following guidelines should be followed on every analysis. Failure to do so can cause damage to the pumps, tubing or your column and will result in the suspension of instrument access.

Aqueous mobile phases should be prepared fresh every day and filtered through a 0.2 µm filter. Prepare a concentrated buffer, filter it, than dilute with deionized water.

An inline or pre-column filter capable of 0.5 µm or smaller particle filtration is required, even for flow injection analysis.

Filter samples through a 0.2 µm filter. Centrifugation alone is not enough to remove protein and particles.

Be sure that the 50/50 methanol/deionized water seal wash bottle is filled.

Always use the divert valve to remove salts to waste instead of the source.

Avoid column shock. When starting the pumps with a column attached, ramp up the flow rate using several steps to reach your desired flow rate. When stopping the pumps, observe that the actual pressure is below 100 psi or 7 barr before removing the column or otherwise breaking the connection.

All columns are not created equally; therefore carefully consideration must be taken for column selection.

The auto-sampler injection loop has a volume of 50 µL, meaning that you can inject a range of 0.1 to 25 µL of your sample, or exactly 50 µL.

Although, the pumps are capable of high pressures it is not recommended to run the system for an extended period of time at maximum pressure.

Note conventional HPLC analysis can be conducted as usual, however adherence to the above sample preparation is expected. The expanded capabilities of the pumping system are available to all that choose to use it.

Return to the MMPF

Personal tools