RRC-EMS: Description of EMS
The Electron Microscopy Service (EMS) is a central facility offering access to instrumentation, training and service using scanning (SEM), transmission (TEM) and scanning transmission (STEM) electron microscopy, surface analysis (XPS) and vibrational spectroscopy (Raman). EM instruments and services are located in two laboratories, one on each side of campus. EMS staff have a broad range of experience covering life and materials science specimen preparation, imaging and spectroscopy using electron microscopes and surface analysis using XPS and Raman.
Instrumentation in the EMS includes four TEMs for materials science applications, one TEM for life science applications; two SEMs used by both life science and materials science users, XPS and a Raman Spectrometer. Equipment for life science specimen preparation includes freeze substitution, coating and ultramicrotomy. Equipment for materials science specimen preparation includes coating, slicing, disc cutting, polishing, ultramicrotomy, electrochemical and chemical thinning and ion beam milling.
The materials science TEMs are a JEOL JEM-ARM200CF cold field emission, probe aberration corrected TEM/STEM, a JEOL JEM-3010 TEM, a VG Microscopes HB601UX dedicated STEM and a JEOL JEM-100CX. The JEM-ARM200CF is a 200kV instrument with a lattice resolution of 0.1nm in TEM and a minimum STEM spot size of 0.08nm. It also has a Gatan Enfina which for Electron Energy Loss Spectroscopy (EELS), and an Oxford AZtec EDX system with a X-Max80 SDD detector. There are two Gatan Orius CCD cameras, a 4MP one above the viewing screen and an 11MP one below the viewing screen. The microscope also has five STEM detectors allowing a range of signals to be collected in scanning mode. The JEM-3010 is a 300kV high resolution TEM with a lattice resolution of 0.14nm and is fitted with a 4MP Gatan Orius camera capable of recording at up to TV rate and a Thermo Noran Vantage EDX system. As well as standard holders there are Heating (1000°C) and Cooling (-170°C) double tilt holders available as well as single tilt Tomography, Scanning Probe and Liquid stages. The Life Science TEM is a JEOL JEM-1220. It is a 120kV instrument fitted with a Gatan Erlangshan 11MP CCD camera and has a lattice resolution of 0.2nm.
The two SEMs are a JEOL JSM-6320F Field Emission SEM and a Hitachi S-3000N Variable Pressure SEM. Both are fitted with EDX systems. The JSM-6320F has an imaging resolution of 1.2nm at 15kV and 2.5nm at 1kV and has a Thermo Noran Voyager EDX system. The S-3000N has an imaging resolution of 3.5nm at 25kV in high vacuum mode and 5nm at 25kV in variable pressure mode and has an Oxford Inca system.
The JEM-1220 and JSM-6320F were both installed in 1997, and the JEM-3010 in 1998, as part of a general effort to upgrade microscopy facilities on campus. The S-3000N was purchased in 2000 and the JEM-ARM200CF in 2011.
The surface analyzer is a Kratos AXIS-165. It is fitted with dual Mg/Al X-ray source and a monochromatic Al X-ray source. It has an ion gun for depth profiling and is capable of spectroscopy and imaging with an X, Y resolution of 30µm and depth resolution of 8-10nm.
The Raman Spectrometer is a Renishaw inVia Reflex interfaced to a Leica DM LM optical microscope with a CCD camera. Spectroscopy and imaging with a resolution of 1 micrometer is possible.